8608341
1996
05
24
1996
05
24
2006
11
15
0906-6705
4
6
1995
Dec
Experimental dermatology
Exp. Dermatol.
Cytokine and protease levels in healing and non-healing chronic venous leg ulcers.
342-9
Leg ulcers present a common and recurring problem in older people creating discomfort and distress for the patient and a great cost to the health care services. Cultured keratinocyte grafts have been used by many investigators to stimulate healing of chronic venous ulcers. It has been proposed that they may do this by producing cytokines which modulate the healing process. However, the types and levels of cytokines in the leg ulcer fluid before and during healing are not known. Wound fluid was collected from venous leg ulcers in 18 patients beneath occlusive Tegaderm dressing for 4 to 6 h. The leg ulcers were divided on clinical criteria into 'healing' and 'non-healing'. PDGF-AB, GM-CSF, IL-1 alpha, IL-1 beta, IL-6 and bFGF were measured by ELISA and the levels of IL-1 alpha, IL-1 beta and IL-6 were also measured using biological assays. The effect of leg ulcer wound fluid on fibroblast and keratinocyte proliferation was measured indirectly by 3H-thymidine incorporation and MTT assay. Total protein, albumin levels, fibronectin degrading activity and collagenase activity, both active and latent were measured. No statistically significant differences in the levels of cytokines or collagenase were identified between healing and non-healing leg ulcers in the sample of leg ulcers studied. However, this study does give valuable information concerning the levels of cytokines and collagenase in chronic leg ulcer wound fluid.
Department of Biochemistry and Molecular Biology, University of Leeds, UK.
Harris
I R
IR
Yee
K C
KC
Walters
C E
CE
Cunliffe
W J
WJ
Kearney
J N
JN
Wood
E J
EJ
Ingham
E
E
eng
Journal Article
Research Support, Non-U.S. Gov't
DENMARK
Exp Dermatol
9301549
0
Albumins
0
Cytokines
0
Proteins
EC 3.4.24.-
Collagenases
IM
Aged
Aged, 80 and over
Albumins
metabolism
Cell Division
physiology
Chronic Disease
Collagenases
metabolism
Cytokines
metabolism
Humans
Middle Aged
Proteins
metabolism
Varicose Ulcer
metabolism
pathology
Wound Healing
physiology
1995
12
1
1995
12
1
0
1
ppublish
8608341
15739652
2005
03
02
2005
05
03
2006
11
15
0969-0700
14
2
2005
Feb
Journal of wound care
Proteases and pH in chronic wounds.
59-61
Smith & Nephew Research Centre, York, UK. bryan.greener@smith-nephew.com
Greener
B
B
Hughes
A A
AA
Bannister
N P
NP
Douglass
J
J
eng
Journal Article
Review
England
J Wound Care
9417080
0
Ointments
EC 3.4.-
Cathepsins
EC 3.4.-
Peptide Hydrolases
EC 3.4.21.-
Serine Endopeptidases
EC 3.4.21.20
cathepsin G
EC 3.4.21.36
Pancreatic Elastase
EC 3.4.21.7
Plasmin
EC 3.4.24.24
Matrix Metalloproteinase 2
N
Bandages
Cathepsins
analysis
physiology
Chronic Disease
Humans
Hydrogen-Ion Concentration
Matrix Metalloproteinase 2
analysis
physiology
Ointments
therapeutic use
Pancreatic Elastase
analysis
physiology
Peptide Hydrolases
analysis
physiology
Plasmin
analysis
physiology
Serine Endopeptidases
analysis
physiology
Skin Care
methods
Wound Healing
physiology
Wounds and Injuries
enzymology
34
2005
3
3
9
0
2005
5
4
9
0
ppublish
15739652
3924283
1985
08
15
1985
08
15
2006
11
15
0267-0623
290
6485
1985
Jun
22
British medical journal (Clinical research ed.)
Chronic ulceration of the leg: extent of the problem and provision of care.
1855-6
A postal survey in two health board areas in Scotland, encompassing a population of about one million, identified 1477 patients with chronic ulcers of the leg. Women outnumbered men by a ratio of 2.8:1. The median age of the women was 74 and of the men 67. Seventy two (5%) were hospital inpatients, 174 (12%) were managed jointly by the primary care team and outpatient departments, and 1201 (83%) were managed entirely in the community. Efforts to improve the management of chronic ulcers of the leg should focus on primary health care.
Callam
M J
MJ
Ruckley
C V
CV
Harper
D R
DR
Dale
J J
JJ
eng
Journal Article
Research Support, Non-U.S. Gov't
ENGLAND
Br Med J (Clin Res Ed)
8302911
AIM
IM
Adult
Aged
Chronic Disease
Community Health Services
Female
Hospitalization
Humans
Leg Ulcer
epidemiology
therapy
Male
Middle Aged
Scotland
1985
6
22
1985
6
22
0
1
ppublish
3924283
3756430
1986
10
30
1986
10
30
2004
11
17
0007-1323
73
9
1986
Sep
The British journal of surgery
Leg ulcers: epidemiology and aetiology.
693-6
In a regional Health District with a population of 198,900, 357 patients with 424 ulcerated legs were documented, an overall prevalence of 0.18 per cent. The prevalence of ulceration in the 92,100 aged greater than 40 years was 0.38 per cent. Fifty per cent of the lesions had been present for more than one year, and 62 per cent of patients had never attended any hospital. The patients were stratified by history of peripheral arterial disease. A random sample (100 patients, 193 legs) was examined by ultrasound and photoplethysmography to assess the venous and arterial circulation; 38 per cent had evidence of deep vein involvement and 43 per cent had superficial vein incompetence. An ischaemic element was present either in isolation (9 per cent) or combined with venous disease (22 per cent) in a total of 31 per cent, with a mean pressure index of 0.62 (s.d. 0.14). Simple objective methods of assessment together with dedicated community staff with ready access to specialized facilities can enhance the service provided to patients with leg ulcers.
Cornwall
J V
JV
Doré
C J
CJ
Lewis
J D
JD
eng
Journal Article
ENGLAND
Br J Surg
0372553
AIM
IM
Adult
Age Factors
Aged
England
Female
Humans
Ischemia
complications
Leg
blood supply
Leg Ulcer
epidemiology
etiology
therapy
Male
Middle Aged
Veins
Venous Insufficiency
complications
1986
9
1
1986
9
1
0
1
ppublish
3756430