8608341 1996 05 24 1996 05 24 2006 11 15
0906-6705 4 6 1995 Dec Experimental dermatology Exp. Dermatol. Cytokine and protease levels in healing and non-healing chronic venous leg ulcers. 342-9 Leg ulcers present a common and recurring problem in older people creating discomfort and distress for the patient and a great cost to the health care services. Cultured keratinocyte grafts have been used by many investigators to stimulate healing of chronic venous ulcers. It has been proposed that they may do this by producing cytokines which modulate the healing process. However, the types and levels of cytokines in the leg ulcer fluid before and during healing are not known. Wound fluid was collected from venous leg ulcers in 18 patients beneath occlusive Tegaderm dressing for 4 to 6 h. The leg ulcers were divided on clinical criteria into 'healing' and 'non-healing'. PDGF-AB, GM-CSF, IL-1 alpha, IL-1 beta, IL-6 and bFGF were measured by ELISA and the levels of IL-1 alpha, IL-1 beta and IL-6 were also measured using biological assays. The effect of leg ulcer wound fluid on fibroblast and keratinocyte proliferation was measured indirectly by 3H-thymidine incorporation and MTT assay. Total protein, albumin levels, fibronectin degrading activity and collagenase activity, both active and latent were measured. No statistically significant differences in the levels of cytokines or collagenase were identified between healing and non-healing leg ulcers in the sample of leg ulcers studied. However, this study does give valuable information concerning the levels of cytokines and collagenase in chronic leg ulcer wound fluid. Department of Biochemistry and Molecular Biology, University of Leeds, UK. Harris I R IR Yee K C KC Walters C E CE Cunliffe W J WJ Kearney J N JN Wood E J EJ Ingham E E eng Journal Article Research Support, Non-U.S. Gov't
DENMARK Exp Dermatol 9301549 0 Albumins 0 Cytokines 0 Proteins EC 3.4.24.- Collagenases IM Aged Aged, 80 and over Albumins metabolism Cell Division physiology Chronic Disease Collagenases metabolism Cytokines metabolism Humans Middle Aged Proteins metabolism Varicose Ulcer metabolism pathology Wound Healing physiology
1995 12 1 1995 12 1 0 1 ppublish 8608341
15739652 2005 03 02 2005 05 03 2006 11 15
0969-0700 14 2 2005 Feb Journal of wound care Proteases and pH in chronic wounds. 59-61 Smith & Nephew Research Centre, York, UK. bryan.greener@smith-nephew.com Greener B B Hughes A A AA Bannister N P NP Douglass J J eng Journal Article Review
England J Wound Care 9417080 0 Ointments EC 3.4.- Cathepsins EC 3.4.- Peptide Hydrolases EC 3.4.21.- Serine Endopeptidases EC 3.4.21.20 cathepsin G EC 3.4.21.36 Pancreatic Elastase EC 3.4.21.7 Plasmin EC 3.4.24.24 Matrix Metalloproteinase 2 N Bandages Cathepsins analysis physiology Chronic Disease Humans Hydrogen-Ion Concentration Matrix Metalloproteinase 2 analysis physiology Ointments therapeutic use Pancreatic Elastase analysis physiology Peptide Hydrolases analysis physiology Plasmin analysis physiology Serine Endopeptidases analysis physiology Skin Care methods Wound Healing physiology Wounds and Injuries enzymology 34
2005 3 3 9 0 2005 5 4 9 0 ppublish 15739652
3924283 1985 08 15 1985 08 15 2006 11 15
0267-0623 290 6485 1985 Jun 22 British medical journal (Clinical research ed.) Chronic ulceration of the leg: extent of the problem and provision of care. 1855-6 A postal survey in two health board areas in Scotland, encompassing a population of about one million, identified 1477 patients with chronic ulcers of the leg. Women outnumbered men by a ratio of 2.8:1. The median age of the women was 74 and of the men 67. Seventy two (5%) were hospital inpatients, 174 (12%) were managed jointly by the primary care team and outpatient departments, and 1201 (83%) were managed entirely in the community. Efforts to improve the management of chronic ulcers of the leg should focus on primary health care. Callam M J MJ Ruckley C V CV Harper D R DR Dale J J JJ eng Journal Article Research Support, Non-U.S. Gov't
ENGLAND Br Med J (Clin Res Ed) 8302911 AIM IM Adult Aged Chronic Disease Community Health Services Female Hospitalization Humans Leg Ulcer epidemiology therapy Male Middle Aged Scotland
1985 6 22 1985 6 22 0 1 ppublish 3924283
3756430 1986 10 30 1986 10 30 2004 11 17
0007-1323 73 9 1986 Sep The British journal of surgery Leg ulcers: epidemiology and aetiology. 693-6 In a regional Health District with a population of 198,900, 357 patients with 424 ulcerated legs were documented, an overall prevalence of 0.18 per cent. The prevalence of ulceration in the 92,100 aged greater than 40 years was 0.38 per cent. Fifty per cent of the lesions had been present for more than one year, and 62 per cent of patients had never attended any hospital. The patients were stratified by history of peripheral arterial disease. A random sample (100 patients, 193 legs) was examined by ultrasound and photoplethysmography to assess the venous and arterial circulation; 38 per cent had evidence of deep vein involvement and 43 per cent had superficial vein incompetence. An ischaemic element was present either in isolation (9 per cent) or combined with venous disease (22 per cent) in a total of 31 per cent, with a mean pressure index of 0.62 (s.d. 0.14). Simple objective methods of assessment together with dedicated community staff with ready access to specialized facilities can enhance the service provided to patients with leg ulcers. Cornwall J V JV Doré C J CJ Lewis J D JD eng Journal Article
ENGLAND Br J Surg 0372553 AIM IM Adult Age Factors Aged England Female Humans Ischemia complications Leg blood supply Leg Ulcer epidemiology etiology therapy Male Middle Aged Veins Venous Insufficiency complications
1986 9 1 1986 9 1 0 1 ppublish 3756430